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New developments in Systems Biology, OMICS-es, Synthetic Biology, Bioinformatics, Automatics, Computer Science, and some crossdisciplinary fields like Cognitive Science, Artificial Intelligence for dealing with biological applications, are morphing into a new subject: Integrated Biotechnology. Integrated Biotechnology focuses strongly on the high level “global behavior” of complex systems, which is considered to be more holistic than reductionistic. Integrated Biotechnology is an interdisciplinary in biotechnology that integrates various disciplines, including genetic engineering, cell culture, bioseparation, mathematics and modeling, bioinformatics, etc, and techniques related to up-, mid-, and down-stream biotechnology, into new one to produce or modify biological products or processes for improving people’s living standards. Integrated biotechnology is unique in bringing together a wide range of scientists, technologists and engineers from so many disciplines as biology, molecular biology, genetics, microbiology, bioinformatics, mathematics and modeling, bioseparation, computer science, engineering principles and so on, particularly in the application of holistic strategy of the up-, mid-, and down-stream biotechnology. This book provides readers with guidance on how to study a complex biological system by taking integrative strategy and how to apply integrated biotechnologies to obtain biological products with some successful examples. Equipped with the knowledge about the foundations and tools of integrated biotechnology, it will become practicable for us to do optimal design and development of bioprocesses from upstream to downstream. This book is a comprehensive textbook of modern biotechnology, the intended readers are graduate students plus academic and professional researchers of life science, medical science, environmental science, food science, agricultural science, bioengineering and biotechnology and so on.
- Table Of Content
Title Integrated Biotechnology Fundamentals and Applications Table of Content Preface. Acknowledgements. About the Authors. PREAMBLE. What is Life: The universe and vitalism: Space, time and universe. Vitalism. Life is an integrate body of matter and spirit: Matter, spirit and life. Several issues on life. Reductionism and Holism: Philosophy and science: Reductionism: Types of reductionism. Reductionism in philosophy and science. Reductionism and life sciences. Limitations of reductionism. Holism: Types of holism. Holism in philosophy and science. Holism and life sciences. Limitations of holism. Holistic and reductionistic approach. What’s Integrated Biotechnology?: Science, engineering and technology. Modern life sciences. What’s Integrated Biotechnology?. Aims, scopes, and roles of Integrated Biotechnology. Perspectives. References. PART I: FOUNDATIONS IN INTEGRATED BIOTECHNOLOGY: Genetic Engineering: DNA replication and gene transcription: Characteristics of the genetic material. DNA replication and synthesis. Gene transcription. Protein and its biosynthesis: Protein structure. Protein synthesis/translation. Metabolism: Bioenergetics. Carbohydrate metabolism. Lipid metabolism. Amino acid metabolism. Nucleotide metabolism. Biological nitrogen fixation. Biosynthesis of typical secondary metabolites. Metabolome. Cell Culture and Fermentation: Metabolic regulatory mechanism: Regulation of enzyme activity. Regulation of enzyme biosynthesisý. Signal transduction systems. Global regulatory controls. Regulation of energy charge. Osmotic regulation. Basic approaches in metabolically controlled fermentation. Microbial growth and environmental factors: Bacterial growth curve. Nutritional types of microorganisms. Nutrients and culture media. Effect of environmental factors on microbial growth. Microbial fermentation: Principles of fermentation technology. Classic fermentation technology. Functional integration techniques. Unit operation integration techniques. Parameter control of fermentation processes: Control of dissolved oxygen. Temperature control. Control of pH. Antifoam control. Bioseparation: Introduction: The characteristics in downstream bioprocess. The RIPP scheme. Cell disruption: High-shear mechanical methods. Sonication. Detergent methods. Enzymatic lysis. Osmotic shock. Other cell disruption methods. Precipitation: Centrifugation. Salting out/salting in. Isoelectric precipitation. Precipitation with organic solvent. Non-ionic hydrophilic polymers as precipitants. Heavy metal salts/polyvalent metallic ions as precipitants. Flocculation by polyelectrolytes. Adsorption: Adsorption mechanism. Adsorption isotherm. Adsorption enthalpy. Adsorption in solution. Adsorbent. Solvent extraction: Water-organic solvent extraction. Aqueous two-phase extraction. Supercritical fluid extraction. Normal and reverse micelles. Chromatographic separation: General chromatography theory. Ion exchange chromatography. Hydrophobic interaction chromatography. Reverse phase chromatography. Affinity chromatography. Size exclusion chromatography. Thin layer chromatograph. High performance liquid chromatography. Expanded bed adsorption. Membrane separation/filtration: Microfiltration. Ultrafiltration. Nanofiltration. Reverse osmosis. Electrodialysis. Drying: Vacuum freeze drying. Pneumatic drying. Spray drying. Fluidized-bed. Crystallization: Crystallization process. Purification. X-ray analysis of crystals. Integration in downstream bioprocessing. References. PART II: TOOLS IN INTEGRATED BIOTECHNOLOGY: Modern Biotechniques: Recombinant DNA technology: The polymerase chain reaction. Molecular cloning. Conventional detection and analytical technology: Gel electrophoresis. Blotting analysis. The study of gene expressions: Microarrays. RNAi technology. Two-hybrid screening. Phage display. The structural analysis of biomacromolecules: X-ray crystallography. Nuclear magnetic resonance. DNA sequencing: Maxam-Gilbert sequencing. Chain-termination method- First generation sequencing. Next generation sequencing. Third generation sequencing. Bioinformatics: Storage and analysis of sequence data: Primary sequence databases and their applications: Sequence alignment and searching: Gene prediction: Genome-wide sequence analysis: Structural bioinformatics. Protein structure databases: Protein structure prediction: Bioinformatics in Transcriptomics. Technologies for large-scale gene expression: Computational analysis of the SAGE data: Computational analysis of the microarray data: Bioinformatics in Proteomics. Experimental workflow of proteomics: Proteomics databases: Protein network analysis: Bioinformatics in Metabonomics. Molecular interaction systems related metabolic pathways: Metabonomics profiles: Final Words: References: PART III. APPLICATIONS OF INTEGRATED BIOTECHNOLOGY. Microbial Cell Factories-Functional Integration. Strategies applied in microbial cell factories. Empirical approaches-fermentation optimization: Genetic approaches-metabolic engineering: Cybernetics: Prokaryotic cell factories-heterologous protein production in Bacillus subtilis. Properties of Bacillus subtilis: Secretory processes of proteins: Genetic engineering approaches: Prokaryotic cell factories-lactic acid fermentation in L. Lactis. Properties of LAB: Lactose metabolism in LAB: Genetic tools for LAB: Metabolic engineering of exo-polysaccharides production: Eukaryotic cell factories-Filamentous fungi. Common strategies used in Filamentous fungi: Production of fungal enzymes: Eukaryotic cell factories-Yeast cells. Lab on A Chip-Unit Operation Integration. Essential components in microfluidics devices. Sample extractor: Mixer: Pump: Valve: Microfabrication techniques. Photolithography: Soft lithography: Stencil Patterning: Applications of LOCs. LOC for analysis of genomics and proteomics: LOC for cell-based assays: Advantages and disadvantages of LOCs: High-throughput Screening in Biopharmaceutical Industry. High-throughput screening. Experimental HTS: Virtual HTS: Protein screening. cDNA library construction: Conversion of cDNA library to protein library: HTS assay development: A captivating biopharmaceutical research paradigm Systems biology. An iterative paradigm linking computer science and biology: A Disrupting paradigm for the traditional notion of drug discovery research References: Subject Index Page No. 489 Author's Name Wang-Yu Tong, Xue-Cheng Zhang, Shan-Qun Jiang and Xiang Dong Publisher Studium Press LLC Year Of Publication 2013 ISBN 10 1-933699-70-1 ISBN 13 No About The Book No Binding type No Book Size(len) No Series No book size(hei) 9 Book size width 6 Edition 1st
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