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This book imparts an enriching experience to the readers by providing extensive information about the techniques in biotechnology. The fundamentals in this book are critical to the success of researchers and students alike. This book covers techniques of DNA isolation, nucleic acid hybridization, RNA isolation, PCR, molecular marker techniques, Immunotechniques, spectroscopy and other techniques applied in biotechnology. The book not only provides the protocols for various techniques but also gives theoretical information regarding it. The principle and working of each technique clarifies the concepts and enhances understanding of the subject. Step-by-step protocols are provided along with preparations of solutions and buffers. Numerous illustrations and diagrammatic representations of the protocol add pictorial value to the theoretical explanation. Troubleshooting guide is an invaluable tool and unique feature of this book, which will help researchers in avoiding pitfalls and obtaining targeted results. Review questions at the end of each chapter, is one more reader friendly unique feature, which will be useful for the teachers, students and researchers alike. The topics covered in this book are in accordance with the postgraduate syllabi of UGC recognized universities and institutions. This book will also be helpful for those who are preparing for competitive exams like NET, JRF, GATE, SET, SRF etc., and for the ones who are engaged in biotechnology research.
- Table Of Content
Title Handbook of Techniques in Biotechnology Table of Content Preface. Electrophoresis: Introduction. Principle and theory of electrophoresis. Applications of electrophoresis. Agarose gel electrophoresis. SDS-PAGE electrophoresis. 2-Dimensional gel electrophoresis. Pulsed field gel electrophoresis. Isoelectric focusing. Review questions. References. Techniques of Centrifugation: Introduction. Theory and principle of centrifugation. Types of centrifuges. Types of centrifugation techniques. Review questions. References. Spectroscopic Techniques: Introduction. UV spectroscopy. Nuclear magnetic resonance (NMR). X-ray spectroscopy. Mass spectroscopy. Review questions. References. Deoxyribonucleic Acid and Extraction of DNA: What is DNA? Its structure and properties. Enzymes that degrade DNA. Presence of DNA in prokaryotic and eukaryotic cell. Composition of cell wall and cell membranes. Enzymes that degrade cell wall. Method for lysing cell membranes and degrading cell walls. Protocols for extraction and purification of DNA. Methods for detection of isolated DNA. Review questions. References. Ribonucleic Acid and Extraction of RNA: What is RNA? Its structure and properties. Enzymes that degrade RNA. Types of RNA 140. Points to remember while isolating mRNA. Creation and maintenance of RNase free working Environment. Protocols for extraction and purification of RNA. Methods for detection of isolated RNA. Review questions. References. Radioisotopes, Radiolabelling of Probes and Autoradiography: Introduction - use of radioisotopes in biological sciences. Radioactive isotopes. Health hazards of radioactivity. Precautions and care to be taken while handling radioisotopes. Radiolabelling of probes. Technique of autoradiography. Review questions. References. Immunotechniques: Introduction: Theory and principle of antibody based detection methods. Extraction and purification of proteins. Enzyme linked immunosorbent assay (ELISA). Radioimmunoassay. Western hybridization. Review questions. References. Techniques of Nucleic Acid Hybridization: Introduction. Use of probes in nucleic acid hybridization. Commonly used solutions and buffers in nucleic acid Hybridization. Southern hybridization. Northern hybridization. In situ hybridization. Colony hybridization. Fluorescent In situ hybridization. DNA microarray. Review questions. References. Techniques of Chromatography: Introduction. Commonly used terms in chromatography. Theory of chromatography. Paper chromatography. Thin layer chromatography. Affinity chromatography. Ion exchange chromatography. Size exclusion chromatography. Gas chromatography. High pressure liquid chromatography. Review questions. References. Polymerase Chain Reaction (PCR): Introduction: Theory and principle. Pre-requisites of PCR- enzymes, dNTPs, primers. What is primer? Comparing primer, probe and gene. Comparison of PCR reaction with in vivo DNA replication process. Applications of PCR. Native PCR. RT- PCR. Review questions. References. Molecular Marker Techniques: Introduction. Importance and applications of molecular marker Techniques. Restriction fragment length polymorphism (RFLP). Random amplified polymorphic DNA (RAPD). Amplified fragment length polymorphism (AFLP). Simple sequence repeats (SSR). Single nucleotide polymorphism (SNP). Sequence characterized amplified region (SCAR). Review questions. Reference. Subject Index Page No. 379 Author's Name Rashmi Ranade & Prof. Sanjay Deshmukh Publisher Studium Press India Pvt Ltd Year Of Publication 2013 ISBN 10 No ISBN 13 978-93-80012-55-1 About The Book No Binding type No Book Size(len) No Series No book size(hei) 9.4 Book size width 6 Edition 1st
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